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Yes, Spatial transcriptomics and scRNA-Seq (single-cell RNA sequencing) are indeed related.
Single-cell RNA sequencing (scRNA-seq) has become essential for biomedical research over the past decade, particularly in developmental biology, cancer, immunology, and neuroscience. Most commercially available scRNA-seq protocols require cells to be recovered intact and viable from tissue. However, this process largely destroys the spatial context that could otherwise inform analyses of cell identity and function.
Spatial transcriptomics is a method that facilitates spatially resolved, high-dimensional assessment of gene transcription. It records the locations of hybridized mRNA molecules in tissue, images the positions of cells themselves prior to assessment, or employs spatial arrays of mRNA probes of pre-determined location.
The integration of spatial transcriptomics and scRNA-seq provides comprehensive insights into spatiotemporal patterns, marker genes, cellular interaction networks, and developmental trajectories. For example, single-cell spatial transcriptomic technology complements the cell-space location information lost during single-cell sequencing. This combined approach is improving our understanding of human tissues in research, diagnostic, and therapeutic settings.
References:
(1) An introduction to spatial transcriptomics for biomedical research …. https://genomemedicine.biomedcentral.com/articles/10.1186/s13073-022-01075-1.
(2) Advances in spatial transcriptomics and related data analysis …. https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-023-04150-2.
(3) Frontiers | ScRNA-seq and spatial transcriptomics: exploring the …. https://www.frontiersin.org/articles/10.3389/fcvm.2023.1064949/full. -
I received this invitation by email:
Comprehensive Quality Control and Interactive Analysis of Single Cell RNA-seq Data. In this webinar, Joshua Campbell will talk about the fundamentals of scRNA-seq analysis and how to handle various challenges.
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A reader may like to know the story from the beginning:
Single-cell sequencing – Wikipedia
Spatial transcriptomics – Wikipedia
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