[A. Diaa]

The two techniques: gene knockout and knockdown, are methods to investigate the functions of different genes. Gene knockout completely makes the gene inoperative while gene knockdown silences the gene of interest. However, both procedures prevent the gene expression. Different agents are capable of accomplishing these two processes. Gene knockout is a type of deletion while gene knockdown is a type of inactivation.

[A. Diaa]

If you give your sample according to instructions, how can it be contaminated by other human DNA?

[A. Diaa]

Transposable elements (TEs) or transposons are defined as DNA sequences that are able to move from one location to another in the genome. TEs have been identified in all organisms, prokaryotic and eukaryotic, and can occupy a high proportion of a species’ genome. For example, transposable elements comprise approximately 10% of several fish species, 45% of the human genome, and up to >80% of the genome of some plants like maize.

DNA transposons consist of a transposase gene that is flanked by two Terminal Inverted Repeats (TIRs). The transposase recognizes these TIRs to perform the excision of the transposon DNA body, which is inserted into a new genomic location.

DNA Transposons: Nature and Applications in Genomics – PMC (nih.gov)

[Author]

Posts