@hammouda
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You should not go for the complementary strand.
The DNA coding strand has the same sequence like mRNA except that U is replaced by T.
Please examine the attached file showing a table of genetic code (codons).
Look at your coding DNA strand, starting from 5′-end find the first ATG sequence that represents methionine codon and the first amino acid in sequence. Count the triplets (codons) which follow this starting codon until you reach a stop (termination), which is not counted.
The attached table shows that termination codons are UAA, UAG, and UGA. In the DNA coding strand, these are TAA, TAG, and TGA.
Good luck.
Actually, the axolotl (Ambystoma mexicanum) was previously known for having the largest genome among animals with 32 billion base pairs. However, recent research shows that the Australian lungfish (Neoceratodus forsteri) has replaced the axolotl as holding the record for the “largest genome in the animal kingdom.
The Australian lungfish (Neoceratodus forsteri) has the largest genome of any animal sequenced so far with 43 billion base pairs. This is around 14 times larger than the human genome.
Reference: Giant lungfish genome elucidates the conquest of land by vertebrates
Most probably you are misinterpreting your results. Many of those who go for the direct-to-consumer DNA testing lack basic knowledge about the human genome. Many do not realize that one person’s DNA is less than 0.1%. different from that of another person. Getting their knowledge from the media and science fiction, they think they have got a quite unique DNA. I have encountered several questions led by this belief. It is the responsibility of the providing companies to educate their consumers to minimize such questions.
This answer is from Quora:
Digital twinning is a technology that involves creating a digital replica or “twin” of a real-world system or process, such as a biological or medical system. Digital twinning allows researchers and practitioners to study and analyze the behavior and performance of a system in a virtual environment, using data collected from the real-world system.
The so called “warrior gene” is the R2 variant of MAOA gene. This variant is of low activity, meaning that it produces less of the protein “monoamine oxidase”. This protein is an enzyme that catalyzes inactivation of the neurotransmitters catecholamines and serotonin. Therefore, low activity of the gene is postulated to cause an increase of the levels of dopamine and norepinephrine. Some studies have linked this condition to increased aggression upon provocation.
The pitfall here is declaring aggressors to be not responsible for their action. Lawyers could use this argument in favor of their clients who committed violent crimes. In a 2009 criminal trial in the United States, an argument based on a combination of “warrior gene” and history of child abuse was successfully used to avoid a conviction of first-degree murder and the death penalty.
https://qr.ae/prpiJlThat is right, especially when using lentivirus for delivering the gene of interest to the host cell. Lentivirus is a retrovirus (RNA that is integrated as its complementary DNA in the host genome). Random insertion of the virus in the host genome may cause insertion mutagenesis. An oncogene may be activated and cause cancer. Early trials for treatment of boys with X-linked severe combined immune deficiency showed the development of some leukemias (blood cancers). Therefore, stringent safety studies should be conducted for new gene therapies, and the patients should be monitored for years after the treatment.
By gene therapy, a new functional gene is introduced into the body cells. It produces the protein that was supposed to be produced by an innate gene. Due to the fact that the innate gene is missing, non-functional, or malfunctional, gene therapy becomes a must.
As with any medical procedure, some complications may arise. The body may react adversely to the newly produced protein, considering it a foreign protein. This may lead to failure of gene therapy, with immune reaction of the body against the produced protein.
Complications may arise from the gene-delivering vector. A common type of vector used is adeno-associated virus (AAV). This vector is used to carry the required gene to the target cells. Although the manufacturers ensure that the virus used is infective but not pathogenic, complications still are encountered.
Guidelines should be followed before, during, and after gene therapy. However, this does not guarantee a zero per cent complications. Recently, we have had a shocking news of two fatalities resulting from gene therapy of spinal muscular atrophy:
Death after Zolgensma – Two Cases Acknowledged – Bioinformatics Hub
This $2.1 million gene therapy is the promising life-saving one-time gene therapy for patients with SMA. Thousands of patients have been successfully treated according to the manufacturer, Novartis
The bottom line is: Medicine is not Mathematics.
Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It provides a method for sorting a heterogeneous mixture of cells into two or more containers, one cell at a time, based on the specific fluorescent characteristics of each cell.
As in ordinary flow cytometry, the cells are passed in a narrow stream of fluid and subjected to a laser beam, one at a time. The stream is then divided into droplets, each containing one cell. The cells are pretreated with fluorescence-tagged antibodies against specific cell surface antigens. A cell with the specific surface antigen will give a specific fluorescence. Once the fluorescence is detected, a charger will give the droplet a positive or a negative charge. The charged droplets are deflected in an electric field towards a specific container. Thus the heterogeneous cell mixture is separated into two or more containers according to the cell surface antigen type.
This technique is useful for research in the fields of cell biology and oncology.
